Composite

Part:BBa_K1675005:Design

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-10)


J23119+B0034+NhaB(BBa_K1675001)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1064
    Illegal AgeI site found at 1376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutation of CTG to CTA Leu.(site:383bp); mutation of GCT to GCG Ala.(site:461bp); mutation of ACC to ACT Thr.(site:566bp); mutation of GAC to GAT Asp.(site:572bp); mutation of ACC to ACT Thr.(site:677bp); mutation of GTA to GTT Val.(site:692bp); mutation of GCA to GCG Ala.(site:1040bp); mutation of GAG to GAA Glu.(site:1067bp); mutation of GTC to GTA Val.(site:1499bp)


Source

The sequence of promoter J23119 is found through the standard part BBa_J23119 https://parts.igem.org/Part:BBa_J23119 The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 https://parts.igem.org/Part:BBa_B0034 The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true

References